Pdf universal protocol for generating 100bp size standard for. Ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100 bp increments, and an additional fragment at 2,072 bp. Invitrogen trackit 100 bp dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. It consists of 15 bluntended fragments at 100 bp increments and is suitable for. By the use of these products you accept the terms and conditions of all. Band 600 bp two to three times brighter than other bands in ladder. Consists of 15 fragments in 100bp increments ranging in size from.
How to dilute dna ladders andersen lab, 2016 invitrogen 1 kb. Life technologies corporation andor it affiliates warrant their. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most poplar markers used for dna fragment size determination. The 10 regularly spaced bands, ranging from 100 bp to 10 bp, with a doublet band at 300311 bp and higher intensity reference bands at 10 bp allow for easy identification and orientation of your band at a glance. Suitable for sizing doublestranded dna from 100 to 1,500bp. This readytouse format reduces handling steps and saves time. Consists of 15 fragments in 100bp increments ranging in size from 1001,500bp and additional band at 2,072bp.
How to dilute dna ladders andersen lab, 2016 invitrogen 1. The 600 bp band is approximately 2 to 3 times brighter than the other ladder bands to provide internal orientation. The 100 bp dna ladder consists of individual chromatography. It consists of 15 bluntended fragments at 100 bp increments and is suitable for sizing doublestranded dna from 100 to 1,500. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. Plus dna ladder if you need size markers for fragments between 100 bp and 12 kb. The 100 bp dna ladder consists of individual chromatographypurified dna fragments ranging in size from 100 bp to 2,000 bp. Quickload 100 bp dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. The migration of tracking dyes is indicated in the figure below.
The 100 bp dna ladder contains 11 discrete dna fragments ranging in size from 100 bp to 3,000 bp. Generuler 100 bp plus dna ladder catalog number sm0321, sm0322 pub. Invitrogen trackit 100 bp dna ladder 100 applications. Our pcrsizer contains ten discrete fragments ranging from 100 bp to. The readytouse 100bp ladder, premixed with loading buffer dye, ranges from 1,500 to 100 bp and has intense reference bands at 1500bp and 500 bp. The dna ladder consists of proprietary plasmids which are. The 100 bp dna ladder consists of 15 bluntended fragments between 100 and. The 10 regularly spaced bands, ranging from 100 bp to 10 bp, with a doublet band at 300311 bp and higher intensity reference bands at 10 bp allow for easy identification and orientation of your band. Agarose gel picture showing the commercial ladder lane m 1kb plus ladder invitrogen lane m1 100bp ladder neb and lane minhouse. The 1650 bp band contains approximately 8% of the mass applied. For technical questions about this product, call the invitrogen techlinesm.
Directload pcr 100 bp low ladder readytouse marker for. Sigmas directload pcr 100 bp low ladder contains 10 dsdna fragments from 100. Three reference bands at 2,000 bp, 1,500 bp, and 600 bp are included for easy. Contents amount storage sm0321 generuler 100 bp plus dna ladder 50 g for 100 applications, 0.
Pcr sizer 100 bp dna ladder the norgen pcrsizer 100 bp dna ladder is prepared to ensure quality and batchtobatch consistency. Application suitable for size determination of pcr generated dna fragments with either agarose or polyacrylamide gels. Dna molecular weight marker xiv 100 bp ladder sigma. Comparison between 1 kb plus dna ladder and 1 kb dna ladder. Quick load 100 bp dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. The 500 bp and 1,500 bp bands contain more dna to provide internal orientation. Generuler 100 bp dna ladder thermo fisher scientific. The bands of the ladder contain fragments following sizes. Our pcr ranger dna ladder contains eleven discrete fragments ranging from 50 bp to. This smobio dna marker dm2200 contains 1 enhanced band 500 bp for easy identification of bands. Making 1kb ladder working solution university of san diego.
The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm. The 100 bp dna ladder is formulated to run accurately and to provide crisp band patterns. Invitrogen trackit 100 bp dna ladder fisher scientific. The gel was visualized and imaged on a uv transilluminator equipped with a camera. Tridye 100 bp dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.
Invitrogen 100 bp dna ladder available on gsava contract for federal government customers only. Directload pcr 100 bp low ladder readytouse marker for dna. Invitrogen 100 bp dna ladder 50ug invitrogen 100 bp dna. The doublestranded dna ladder can be visualized on 4% to 5% agarose gels for 10bp to 150bp fragments, 2% to 3% agarose gels for 150bp to 330bp fragments, 2% to 3% agarose gels, or 6% to 10% polyacrylamide gels after ethidium bromide staining. The 600 bp band is approximately 2 to 3 times brighter. The 10bp dna ladder consists of 33 ten base pair repeats. If samples need to be diluted, use te or other buffer of minimal ionic strength. The tracking dyes do not obscure visualization of dna bands in ladder as the dyes run outside the limits of most dna bands in ladder. The 500 bp band with higher concentration is distinguished easily from. Trackit 100 bp dna ladder thermo fisher scientific. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended.
Trackit 100 bp dna ladder is premixed with sample buffer for readytoload convenience and room temperature storage. Can be visualized on 1% to 2% agarose gels after ethidium bromide staining. Ladder should be loaded in a single lane on an agarose gel. The invitrogen trackit 100 bp dna ladder is designed for sizing and quantification of double stranded dna on 1% to 2% agarose gels. Agarose gel analysis shows that the bands between 100 bp and 1,500 bp are accurate in size and distinguishable. Smobio accuband 100 bp dna ladder ii dm2200 is composed of 10 individual dna fragments, presenting 1. The intensity of 500 bp is about three times than the other bands to serve as reference points. Identification of the preparationsubstance and of the company preparation name. The 10 regularly spaced bands, ranging from 50 bp to 2000 bp, with higher intensity reference bands at 300 bp, bp and 2000 bp allow for easy identification and orientation of your band at a glance. The 100 bp dna ladder consists of individual chromatographypurified. The norgen pcr ranger 100 bp dna ladder is prepared to ensure quality and batchtobatch consistency.
Invitrogen 25 bp dna ladder suitable for sizing doublestranded dna from 25 bp to 500 bp. Our pcrsizer contains ten discrete fragments ranging from 100 bp to bp in 100 bp increments with a higher intensity reference band at 500 bp. Neb also offers a quickload version of this ladder with purple dye. Dna ladder 100 bp and dna ladder 1kb marker for dna rna agarose gel electrophoresis. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. Dna molecular weight marker xiv 100 bp ladder allows accurate sizing of dna fragments generated by pcr or restriction digest or rtpcr, following separation on agarose gels. The 500 bp band with higher concentration is distinguished easily from others. The invitrogen 100 bp dna ladder is designed for sizing and quantification of double stranded dna on 1% to 2% agarose gels.
The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm tris ph 7. Dna molecular weight marker xiv 100 bp ladder sigmaaldrich. Invitrogen 25 bp dna ladder 50ug nucleic acid molecular. The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of gel loading dye, purple 6x, no sds. Invitrogen 10 bp dna ladder 50ug nucleic acid molecular. Use the neb 1 kb ladder for size markers between 500 bp and 10 kb. The gel was visualized and imaged on a uv transilluminator. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp.
The 100 bp dna ladder is a readytoload molecular weight marker suitable for dna fragment size determination on gel electrophoresis. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. This marker is ideal for the size determination of pcr products. The invitrogen 100 bp dna ladder is designed for sizing and quantification of double stranded. The dna molecular weight marker can be used in conjunction with dna molecular weight marker xiii 50 bp ladder for precise size determination. The 100 bp dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to bp. The dna ladder consists of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis.
The 100 bp plus dna ladder consists of 19 double stranded molecular weight. Accuband 100bp dna ladder ii, dm2200, 500ulvial, 200 loads. Thermo scientific generuler 100 bp dna ladder is designed for sizing. Readytoload 100 bp plus dna ladder ranging from 100 to. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp.
The norgen pcrsizer 100 bp dna ladder is prepared to ensure quality and batchtobatch consistency. General description sigmas directload pcr 100 bp low ladder contains 10 dsdna fragments from 100. Generuler 100 bp plus dna ladder thermo fisher scientific. The 100 bp dna ladder consists of 15 bluntended fragments between 100 and 1500 bp in multiples of 100 bp and an additional fragment at 2072 bp. The 100 bp dna ladder is a unique combination of a number. Our pcr ranger dna ladder contains eleven discrete fragments ranging from 50 bp to bp with a higher intensity reference band at 500 bp. Store at 25c for 6 months store at 4c for 12months for long term storage, store at 20c recommended pdf data.
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